Colorectal Cancer: Methods and Protocols by Steven M. Powell

By Steven M. Powell

Instructs investigators in key genetic, mobile, and molecular organic equipment of studying colorectal tumors. suggestions and assays are defined intimately to permit researchers to scan on colon tumors. so much chapters are written through scientists who pioneered the equipment offered. insurance contains gross microdissection of colon tumors, institution of mobile traces and xenografted tumors, chromosomal analyses similar to FISH assays, mutational analyses of colon tumors and blood samples, gene expression analyses, and microsatellite instability trying out. contains a few colour pictures. For researchers at a number of degrees, from graduate scholars to pros. Powell is affiliated with the college of Virginia health and wellbeing procedure.

Show description

Read or Download Colorectal Cancer: Methods and Protocols PDF

Similar digestive organs books

Portal Hypertension III

Complaints of the 3rd Baveno overseas Consensus Workshop on Definitions, technique and healing ideas. The members tried to increase consensus definitions of key occasions in portal high blood pressure and variceal bleeding, diagnostic and healing concerns, and the issues of treatments for portal high blood pressure, in addition to generating up-to-date directions.

Psychological Factors Affecting Medical Conditions: A New Classification for DSM-V

In DSM-IV, the relationships among actual ailment and mental components are grouped below the classifications 'Somatoform issues' and 'Psychological components Affecting clinical Conditions'(PFAMC). This quantity introduces the 'Diagnostic standards for Psychosomatic learn' (DCPR) as a brand new suggestion for the PFAMC component of DSM-V.

Gastroesophageal Reflux Disease - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References

It is a 3-in-1 reference publication. It supplies an entire scientific dictionary overlaying thousands of phrases and expressions when it comes to gastroesophageal reflux sickness. It additionally supplies large lists of bibliographic citations. eventually, it presents details to clients on the best way to replace their wisdom utilizing numerous web assets.

Pediatric Gastroenterology: A Color Handbook (Medical Color Handbook Series)

Hugely counseled, BMA scientific publication Awards 2015During the previous twenty years, there was an explosion of scientific, simple technology, and translational examine resulting in a greater figuring out of the body structure and disorder approaches within the gastrointestinal procedure of youngsters. Endoscopic recommendations have more desirable, correlation of radiographic and biopsy findings with disorder became higher outlined, and advances in transplant care have resulted in markedly greater survival, even within the smallest of babies.

Extra info for Colorectal Cancer: Methods and Protocols

Sample text

3. Stop the reaction by heating the Eppendorf tube at 70°C for 10 min. 4. Add 400 ng (800 ng for paraffin-embedded tumors) of each of both labeled DNAs (tumor and reference of the same sex), 20 µg of unlabeled Cot-1 DNA, 10 µL of 3 M Na acetate and 700 µL absolute ethanol. Mix and keep for at least 2 h at –20°C to precipitate the DNA probe. 5. In a microcentrifuge at 4°C, spin the probe for 30 min at 10,000g. Discard supernatant and allow the DNA pellet to dry at 37°C for 20–30 min. 6. The DNA probe is dissolved in 10 µL of hybridization buffer for at least 2 h at 37°C.

Is the most wonderful feature offered by fluorescence in situ hybridization (FISH). DNA in situ hybridization is a technique that allows the visualization of defined sequences of nucleic acids within the individual cells. The method is based on the site specific annealing (hybridization) of single-stranded labeled DNA fragments (probes) to denatured, homologous sequences (targets) on cytological preparations, like metaphase chromosomes, interphase nuclei, or naked chromatin fibers. Visualization of hybridization sites becomes possible after detection steps by using a wide spectrum of the fluorescent dyes available.

2. Preparation of Metaphase Slides (see Note 2) 1. Adjust the humidity to 70–80% and the temperature to 25°C in a hood. 2. From a distance of 30 cm, drop one to two drops from your fixed cell suspension onto the slide. 3. Keep the slide 5 min inside the humid hood before taking it out. 4. Check your metaphases with a phase-contrast microscope and accept only slides with black spread metaphases. 5. Allow the slides to age at room temperature (25–30°C) for 1–2 wk. 6. Aged slides can be successfully used for another 2 wk.

Download PDF sample

Rated 4.16 of 5 – based on 35 votes