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Additional resources for Detection of Biological Agents for the Prevention of Bioterrorism
It is also evident that fractionation can significantly increase the analytical information obtained from the spectra. The number of characteristic signals of each species found in the spectra from each band always increased with respect to the number of characteristic signals found in the spectra of the unfractionated mixture. 11a and b. 11b, the E. 9). 32 P. Reschiglian et al. 10; §: Most characteristic B. subtilis peaks recovered. 10; #: Most characteristic E. coli peaks recovered. Adapted with permission from ref.
Firstly, possible run-torun sample carry-over due to incomplete sample recovery from F4 could affect spectra reproducibility and, thus, reduce fingerprinting capabilities of MALDITOF-MS. Secondly, the relatively high sample dilution reached after the F4 step could result in cell concentrations that are below the detection limits for MALDITOF-MS. Thirdly, the time required by the F4 step could affect the intrinsic rapidity of MALDI-TOF-MS analysis. When compared to commercial, flat-type F4 technology, potentially disposable usage of HF5 shows not only the advantage to eliminate risks of run-to-run sample contamination, but also the advantage to reduce possible sterilization issues.
Coli and B. subtilis cells. #: Most characteristic E. coli peaks recovered; §: Most characteristic B. subtilis peaks recovered. Adapted with permission from ref. , © 2004, American Chemical Society reported in Table 1 with E. coli identification data reported in the literature,51 and with data obtained from a model-derived protein biomarker search5 makes it possible to assign at least one of these signals to one E. 8). Figure 9 reports a representative MALDI/TOF spectrum obtained by mixing an approximately equal amount of lyophilized E.